User blog comment:Suriken88/Ezio's speculated death in Embers/@comment-110.55.32.43-20120112022832

Once Ezio decided to retire as an Assassin, the two left Maysaf, got married, and moved to a Villa outside Firenze. Their marriage produced two children—Flavia (b. 1513) and Marcello (b 1514). By 1525, Ezio's health was deteriorating, and he eventually died of a heart attack in Firenze. In his final letter to Sofia, he commented that the love he had for Sofia and their children was what kept him going in his old age.