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Ark of the Covenant
The Ark of the Covenant was hidden under Solomon's Temple and housed a Piece of Eden. Altaïr was sent there to retrieve it in the opening of Assassin's Creed. On the mission he broke the three tenants of the Creed when trying to kill Robert de Sable, the Grand Master of the Knights Templar, who was there as well. This nearly caused the Assassins to not get the Piece of Eden and demoted him to from a Master Assassin to a Novice.