Easter eggs

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Mario

Uncle Mario introduces himself outside your Villa, "It's a-me! Mario!" referencing the main menu introduction to Mario 64.

Giant Squid

A giant squid can be found in Santa Maria Delle Visitazione. The squid appears after actuating the lever that brings out the bars to swing and traverse the final chamber. Ezio must wait for about a minute at the waters edge next to the lever.

A video was posted demonstrating this Easter Egg. http://www.youtube.com/watch?v=oJZI-54QmRc

Altair's Costume

When at the main menu, press the Y button to enter Uplay. If you have certain achievements, you can purchase things such as Altair's Costume and extra throwing knives.

Mortal combat

sometimes when you are fighting a group of guards one of them will shout out Finish Him