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Easter eggs
Easter Eggs in Assassin's Creed 2
Mario
Uncle Mario introduces himself outside your Villa, "It's a-me! Mario!" referencing the main menu introduction to Mario 64.
Giant Squid
A giant squid can be found in Santa Maria Delle Visitazione. The squid appears after actuating the lever that brings out the bars to swing and traverse the final chamber. Ezio must wait for about a minute at the waters edge next to the lever.
A video was posted demonstrating this Easter Egg. http://www.youtube.com/watch?v=oJZI-54QmRc