Adam and Eve
thumb|300px|right|The Truth video
Adam and Eve were the two humans shown in Subject 16's The Truth video. They were humans who rebelled against Those Who Came Before. In the Truth video, they are seen running from someone or something. They use free-running to climb a large, futuristic looking building. While Adam and Eve are climbing the building, Eve can be seen holding a stolen Piece of Eden which helps her look through the darkened glass at the human slaves within the building. When they are on top of the building, Eve says: "Adam, I have it!". Adam then says "Eve" and Eve yells: "Look out!". This could be because they see someone or something behind them, or the Apple gave them power enough to realize they were being watched by Subject 16, as it did with Minerva in the Vault. This explains why they did not react until after they had The Apple.
Both Adam and Eve seem to have white markings covering their bodies which occasionally glow blue. These lines do not follow blood vessels, indeed, the markings on the back are straight and angular. Their designs look slightly like circuit boards, and their perfectly angular, symmetrical design makes a natural origin less likely. The designs of these lines also are similar to those of the character Minerva, who Ezio encounters in The Vault, which hints that Adam and Eve could be part of the second generation humans that are a result of the first generation humans that procreated with Those Who Came Before e.g. Minerva, which would explain how Adam and Eve appear to be immune to the controlling power of the Apple. Them being children of Those Who Came Before would also explain the statement "The seeds were planted as two worlds became one, behold, the Assassins, the children of two worlds!" found in a puzzle of the truth. Furthermore, Eve appears to have something attached to her left arm, and attached around her neck, possibly a collar or necklace.
The Binary Code at end of The Truth video translates as:
01000101=E
01000100=D
01000101=E
01001110=N
The couple appear to have disobeyed their masters by stealing a Piece of Eden - specifically, one of the Apples which presumably has given them immunity to hypnotism. The theft of the artifact could have been the first act of defiance which caused the conflict as described by Minerva. The 'innocence' of mankind was lost when they realized that the superiority of Those Who Have Gone Before was due to their technological advances. This prompted a war between the two species. Another possibility is that they were given the artifact by one Those Who Have Gone Before, possibly one who is analogous to the religious entity of Satan. Subject 16's record also shows that the artifacts (the Staff, the Apple, the Pieces of Eden) were tools that the First Civilization used to wield power over humans. Whether the artifacts were made to control humans, or if humans were made to obey the artifacts, is another matter. Even so, some are immune to their effects.
Biblical
Genesis tells the story of Adam and Eve in chapters 1, 2 and 3, with some additional elements in chapters 4 and 5:
Genesis 2 opens with God fashioning a man from the dust and blowing life into his nostrils. God plants a garden (the Garden of Eden) and sets the man there, "to work it and watch over it," permitting him to eat of all the trees in the garden except the Tree of Knowledge of Good and Evil, "for on the day you eat of it you shall surely die." God had already created the animals, and when Adam tried to find a help-mate, none of the animals are satisfactory, and so God causes the man to sleep, and creates a woman from his rib. The man names her "Woman" (Heb. ishshah), "for this one was taken from a man" (Heb. ish). "On account of this a man leaves his father and his mother and clings to his woman." Genesis 2 ends with the note that the man and woman were naked, and were not ashamed.
Genesis 3 introduces the Serpent, "slier than every beast of the field." The serpent tempts the woman to eat from the tree of knowledge, telling her that it will not lead to death; she succumbs, and gives the fruit to the man, who eats also, "and the eyes of the two of them were opened." Aware now of their nakedness, they make coverings of fig leaves, and hide from the sight of God. God asks them about what they have done. Adam blames Eve, and Eve blames the serpent. God curses the snake (who before this must have been able to walk before this because) vs 14 says "upon thy belly shalt thou go, and dust shalt thou eat all the days of thy life." God then curses Adam and Eve with hard labor and with pain in childbirth, and banishes them from his garden, setting a cherub at the gate to bar their way to the Tree of Life, "lest he put out his hand ... and eat, and live forever."
Genesis 4 and 5 give the story of Adam and Eve's family after they leave the garden: three children are named, Cain, Abel and Seth, as well as other sons and daughters. Adam lived for 930 years. ("The woman" is given the name Eve in the closing verses of Genesis 3, "because she was the mother of all living"; Adam gets his name when the initial definite article is dropped, changing "ha-adam", "the man", to "Adam".)