Amelia

Amelia was a woman living in the countryside of Romagna during the Renaissance. In 1481, while the Assassin Ezio Auditore da Firenze was searching for Leonardo and his boat to Venice, he happened across Amelia and two of her friends discussing whether or not someone could beat one of their records in a horse race. Ezio took on the challenge, and upon breaking the record, he returned to collect his prize: a "private riding lesson" from Amelia.
She is most likely intended by the developers to be Clay Kaczmarek's ancestor. She had a one-night stand with Ezio after winning the horse race bet, then afterwards they went their separate ways, never to meet or see eachother again. However, unknowing and uncaring to Ezio, she became pregnant and gave birth to Ezio's firstborn child/children, whose line of descendants would eventually lead down to Clay. This perfectly explains why the Animus showed Clay to be biologically connected to Ezio at the beginning of the game.
Gallery
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Amelia asking Ezio to beat the course record
Appearances
References
es:Amelia fr:Amelia hu:Amelia it:Amelia ru:Амелия uk:Амелія zh:阿梅利亚