His outfit highly resembles the ones worn by doctors during the plague, and appears black in the dark. His face is concealed by a mask, therefore making his true identity unknown. His outfit is different from what the doctors wear in Assassin's Creed II, most noticeably due to the shorter beak of his mask.
Ubisoft has said that he will make an appearance in the single player mode of Assassin's Creed: Brotherhood along with the other multiplayer characters.
He appeared in Project Legacy where it is revealed he is in the employ of Cesare Borgia and is known as a serial killer that targets courtesans. Fiora Cavazza called him a "monster". He drugged her (or poisoned her and tried to kill her) even after she gave him Cesare's document. However, she was able to defend herself, cutting him badly. Whether his attack was ordered by Cesare in the letter, or by Malfatto's own will is unknown.
Ezio came across a courtesan, crying over a fellow courtesan's dead body. She told him Malfatto murdered her, and he was shortly thereafter assassinated by a member of the Brotherhood of Assassins under Ezio's orders.
The Doctor charges at his target from behind and swiftly thrusts his syringe into the target's shoulder, injecting poison into their system. Before the victim can realize what has happened, they collapse to the ground and die silently. The Doctor can also perform a similar move from the front of the victim, where he thrusts the syringe into the victim's neck.
The Eye Stab
The Doctor charges and jumps onto his target, injecting the syringe into their eye and causing death.
Trivia
Malfatto is most likely based off of Jack the Ripper, a well known serial killer that preyed on prostitutes in impoverished areas of London around the year 1888. The removal of internal organs from his victims led many to believe that Jack was a doctor or possessed surgical knowledge.
Malfatto is an Italian surname that may derive from the Italian word malfattore or "someone who does evil deeds."
Oddly enough the only trace of Malfatto in history are reports of a masked doctor sighted near the Rosa in Fiore brothel.
His Advanced Gear changes the eye pieces of his mask.
