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Roanoke Colony
The Roanoke Colony was a late 16th century attempt by Queen Elizabeth I to establish a permanent English settlement in North America. Located on Roanoke Island in present-day North Carolina, United States, the colonists of Roanoke misteriously vanished in the late 1580s, leaving the settlement abandoned.
According to Erudito, the disappearance of the colony had some connection to the Assassin-Templar war.